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Validation of a LC-MS/MS method of Analysis of Nicotinic acid and Nicotinamide in infant formula and adult
nutritional samples
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Copyright © 2012 Silliker, Inc.
7
B3-acid IS2
128.1
54
20
50
10
29
10
B3-acid IS3
128.1
81
20
50
10
28
10
B3-amide IS1
127
80
20
45
10
27
10
B3-amide IS 2
127
82
20
45
10
34
10
B3-amide IS 3
127
55
20
45
10
35
10
MS
_Start.OnDuration=0.5
MS_Start.Off Duration=12.00
Results
A LC-MS/MS method for analysis of nicotinic acid and nicotinamide and calculation of total
niacin (vitamin B3) in infant and adult nutrition formula and pet food matrices was developed
and validated. Results are presented in the following sections.
(a)
Results of Chromatogrphy by the method: The chromatographic part of the method
could separate nicotinic acid and nicotinamide in the samples from other water soluble
B vitamins may present and also from any matrix interferences. Peak shapes of the
nicotinic acid as well as nicotinamide were good (fig 1).
1.) Chromatogram of a Mixture vitamin B Standards – approx 20ppb
VALIDATION REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE