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SMPR CHALLENGES - RECOVERY
• Accuracy/ “Trueness” in gluten analyses has
always been a difficult concept to formally
define and experimentally estimate.
• In the past, barley has always been a minor
consideration for choosing antibodies.
• How to develop criteria to evaluate relative
responses of wheat, rye and barley?
• Criteria for kit response (extraction +
antibody) should be evaluated individually for
3 grains.
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y = 0.2965x + 0.2085
R² = 0.9977
y = 0.2362x + 2.4442
R² = 0.9893
y = 0.1034x + 0.8348
R² = 0.999
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GLUTEN MG/KG
SPIKE MG/KG
GLUTEN R5 Assay Kit Relative Response by Spiked Grain Weight
Barley
Rye
Wheat
Linear (Barley)
Linear (Rye)
Linear (Wheat)
BARLEY SLOPE = 29.7%
WHEAT SLOPE = 10.3%
RYE SLOPE = 23.6%
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