Microsoft Word - LowLactose_ERP-Proposal-May2019

MEMORANDUM 

Date:  

July 24, 2019 

To: 

AOAC INTERNATIONAL Official Methods Board 

From:  

Deborah McKenzie, Staff Liaison – AOAC Official Methods Board 

Subject: 

New Expert Review Panel (ERP) for Low‐Lactose Methods 

BACKGROUND  AOAC has had a Sugars Working Group that developed three standard method performance  requirements (SMPR®): Sugars for Animal, Pet, and Human Food and Ingredients, Fructans  in Animal and Pet Food and Ingredients, and Low‐Lactose in Human Food and Ingredients.    Last summer, the OMB formed an ERP for Sugars and Fructan Methods to review methods  received based on an issued call for methods.  AOAC also issued a call for methods for low‐ lactose methods. Please find below a proposal for an expert review panel (ERP) to review  methods received as a result of a call for methods for that specific SMPR.    A call for experts was issued to solicit applicants to form a low‐lactose ERP.  Nineteen (19)  applications were received with varying levels of experience and expertise.  Table I is a  proposed roster for the ERP based on review of summaries of expertise, CVs, and any  known AOAC related activity.  Of the 19 applications, 14 are presented to form the ERP and  this is enough to formulate an ERP.  Also, there are three (3) applicants who are potential  members if needed and two (2) applicants who did not exhibit qualifications sufficient for  this ERP.    RECOMMENDATION  OMB to review the proposed ERP for Low‐Lactose Methods in Table I using the applicant  information including: Perspectives of Proposed ERP, Table II – List of Applicants,  Perspectives of Applicants, Statements of Expertise with Staff Recommendations, and CVs,  and render a decision on this ERP and verify the status for all applicants.

I.

Proposed Expert Review Panel for Low‐Lactose Methods 

Table 1 ‐ Proposed Roster for AOAC ERP for Low‐Lactose Methods  FIRST  LAST  ORGANIZATION 

POSITION 

1. Sean  2. Lucien  3. David  4. Michael  5. George 

Austin 

Nestle Research 

CHAIR 

Duchateau  Ellingson  Goodrich 

DSM Food Specialties 

MEMBER  MEMBER  MEMBER  MEMBER  MEMBER  MEMBER  MEMBER  MEMBER  MEMBER  MEMBER  MEMBER  MEMBER  MEMBER  POTENTIAL  POTENTIAL  POTENTIAL  NOT AT THIS  TIME  NOT AT THIS  TIME

Eurofins Food Integrity and Innovation  Cornerstone Analytical Laboratories 

Joseph 

AsureQuality 

6. Brian 

Lang 

US NIST  US FDA 

7. Sookwang 

Lee 

8. Hari 

Narayanan 

Metrohm USA Inc 

9. Salvatore  10. Angelika  12. Marina  13. Martine  14. Jinchuan   11. John 

Parisi 

Al Balqa Applied University 

Semmler  Szpylka 

Chr Hansen 

Merieux NutriSciences 

Torres 

LATU 

van Gool 

FrieslandCampina 

Yang 

Waters 

Nour Eddine  Es‐Safi 

Mohammed V University in Rabat   Medical University of Lublin, Poland  First source laboratory solutions LLP 

Tomasz 

Tuzimski  Yadlapalli 

Sudhakar 

Mehmet 

Gumustas 

Ankara University Institute of Forensic  Sciences 

Markus 

Lacorn 

R‐Biopharm 

II.

Perspectives of Proposed ERP 

Broad Perspectives

7%

21%

72%

Academia Government

Industry

Specific Perspectives

11%

11%

36%

7% 7%

28%

CRO Food Production Reference Materials Regulators Research Technology

Regional Perspectives

8% 7%

7%

7% 7%

50%

7%

7%

Belgium Denmark Italy Netherlands New Zealand Switzerland Uruguay USA

III.

Methods Expected for Review  1. Submitted by: Eurofins Nutrition Analysis Center  Title:   Quantitation of Six Common Food Sugars in Various Matrices by HPLC‐MS 2. Submitted by: Eurofins Food, Feed & Water Testing Netherlands  Title:   Lactose, free and low, Profile by High Performance Anion Exchange  Chromatography with Pulsed Amperometric Detection (HPAEC‐PAD)

Final list of submitted methods to be determined ‐ Call for Methods still active. 1

IV.

Low Lactose Expert Review Panel:  2019 Applicants 

Table 2 ‐ List of Applicants Responding to Call for Experts 

Region 

First 

Last 

Organization 

Switzerland 

1 Sean  2 Lucien  3 David 

Austin 

Nestle Research 

Belgium 

Duchateau 

DSM Food Specialties 

USA 

Ellingson 

Eurofins Food Integrity and Innovation  Mohammed V University in Rabat   Cornerstone Analytical Laboratories 

Morocco 

4 Nour Eddine 

Es‐Safi 

5 Michael  6 Mehmet  7 George  8 Markus 

Goodrich  Gumustas 

USA 

Turkey 

Ankara University Institute of Forensic Sciences 

New Zealand 

Joseph  Lacorn 

AsureQuality  R‐Biopharm 

Germany 

USA  USA  USA  Italy 

9 Brian 

Lang 

US NIST  US FDA 

10 Sookwang 

Lee 

11 Hari 

Narayanan 

Metrohm USA Inc 

12 Salvatore  13 Angelika 

Parisi 

Al Balqa Applied University 

Denmark 

Semmler  Szpylka 

Chr Hansen 

USA 

14 John 

Merieux NutriSciences 

Uruguay 

15 Marina  16 Tomasz  17 Martine  18 Sudhakar 

Torres 

LATU 

Poland 

Tuzimski  van Gool  Yadlapalli 

Medical University of Lublin, Poland 

Netherlands 

FrieslandCampina 

India  USA 

First source laboratory solutions LLP 

19 Jinchuan Yang 

Yang 

Waters 

1 https://www.aoac.org/aoac_prod_imis/AOAC_Member/ANews/2018_News/NEWS_122018.aspx

Applicant Perspectives  

V.

Low‐Lactose ERP Applicants ‐ Broad  Perspectives

26%

58%

16%

Academia Government

Industry

Low‐Lactose ERP Applicants ‐ Specific  Perspectives

22%

28%

6%

22%

11%

11%

CRO

Food Production

Instrument Manufacturer

Product Certification

Regulators

Research

Low Lactose Regional Perspective

6% 6% 6% 5%

37%

5%

5%

5%

5%

5% 5% 5% 5%

Belgium Denmark

Germany

India

Italy

Morocco

Netherlands New Zealand Poland

Switzerland

Turkey

Uruguay

USA

VI. Applicant Statements of Expertise & Staff Recommendations (click on name to access CV) Sean Austin  Nestlé  Food  Production  Industry  Switzerland  Statement of Expertise  I have worked for many years on the determination of carbohydrate components in food products.   Currently I am responsible for the development, validation and technical support of methods used  by Nestlé for quality control of their products (including analysis of sugars and lactose in sugar‐free  and/or lactose‐free products)  Staff  Recommendation  Has significant method development and validation in carbohydrate/sugar  analysis in infant formula, dairy, and food products.  Served as a member  of the low lactose working group and serves as a member of the SPIFAN  Nutrients ERP for the FOS and GOS methods, chair of the FOS/GOS  YES – ADD TO THIS ERP  AS POTENTIAL CHAIR  Statement of Expertise  As principal scientist within the analytical department of DSM Food Specialties, I am for more than  10 years involved in the analysis of sugars in different matrices. More specifically, in the analysis low‐ lactose concentrations in several dairy products.   Staff  Recommendation  YES – ADD TO THIS ERP  Has significant experience with method development and analysis of food  and food ingredient.  He’s served a member of the low lactose working  group.    Statement of Expertise  I have a number of years of experience in the carbohydrate realm of analysis, and particularly in  sugars. I have developed our own internal method for low lactose a few years back.    Staff  Recommendation  Has significant method development and validation with analysis of dairy,  food, ingredients, and raw materials.  Also served as a member of the  working group.    David Ellingson  Eurofins Food Integrity  & Innovation  CRO  Industry  USA  working groups in SPIFAN, and serves as a member for Dietary Starch and  Fiber ERP.  Highly recommend and support his nomination to chair the  ERP.    Lucien Duchateau  DSM Food Specialties  Food  Production  Industry  Netherlands 

YES – ADD TO THIS ERP 

Mohammed V  University in Rabat 

Research 

Academia 

Morocco 

Nour Eddine ES‐SAFI 

Statement of Expertise  Chemist with expertise in natural products: extraction, isolation, quantitative and qualitative  analysis.    Staff  Has significant instrumentation experience with analysis of food,  ingredients, and raw materials.  Did not serve on the working group but is  a member of many ERPs.  Could be a member of ERP. 

Recommendation  POSSIBLE ADDITION

Cornerstone Analytical CRO 

Industry 

USA 

Michael Goodrich 

Statement of Expertise  I have over 20 years of chromatography experience and validated methods for EPA, USP and AOAC.   I would appreciate the opportunity to assist in this endeavor.   I have experience with LC and IC

methods for sugars at trace levels.    Staff  Recommendation  YES – ADD TO THIS ERP 

Has instrumentation and method development experience; owns  laboratory for Cornerstone that does sugars and lactose testing.   

Mehmet Gumustas 

Ankara University 

Research 

Academia 

Turkey 

Statement of Expertise  I have expertise on chromatographic methods.  Staff 

Strong analytical chemistry, method validation, and instrumentation  background; however, none specific to sugars or carbohydrate analysis. 

Recommendation  NOT AT THIS TIME 

George Joseph 

AsureQuality 

Product  Certification 

Government 

New Zealand 

Statement of Expertise  Over 25 Years of experience in this area  Staff  Recommendation  YES ‐ ADD TO THIS ERP 

Strong analytical chemistry, method development and validation, infant  formula and dairy analysis, chair of the Sugars Fructans ERP; member of  Dietary Starch and Fiber ERP 

Markus Lacorn 

R‐Biopharm 

Instruments 

Industry 

Germany 

Statement of Expertise  Dear all  One oy my main areas of expertise is the development, validation and production of enzymatic test  kits  Sr. Staff Review  No information on familiarity of lactose or instrumentation other than enzymatic test kits  Staff  Has expertise in method development and method validation of enzymatic  tests, but not for sugars or carbohydrate analysis; however, he was a  member of the sugars working group.  Recommendation  NOT AT THIS TIME 

.  Brian Lang 

NIST 

Product  Certification 

Government 

USA 

Statement of Expertise  I have worked with Ion Chromatography for over 12 years, including measurement and identification  of sugars.  I have used Ion Chromatography with pulsed amperometric detection in the analysis of  various sugars and functionalized sugars at trace levels that are at are below the Standard Method  Performance Requirements.  

Staff  Recommendation  YES – ADD TO THIS ERP

Has method development experience and has published on sugar analysis  He also has significant experience with chromatographic plaforms and  nutritive component analysis.  

Sookwang Lee 

US FDA 

Regulator 

Government 

USA 

Statement of Expertise  Analyzed sugars (including lactose) in regulatory samples with a various types of matrices, both  domestic and imported.  Staff  Has significant expertise in sugars/carbohydrate analysis.  Also was a

Recommendation  YES – ADD TO THIS ERP 

member of the sugars working groups and a member of the Sugar –  Fructans ERP.   

Hari Narayanan 

Methrohm USA 

Instruments 

Industry 

USA 

Statement of Expertise  Expert in Sugar Profile analysis using Liquid Chromatography and Ion Chromatography and sample  preparation.    Staff  Recommendation  Has significant experience in method development and validation in sugars  and dairy analysis; Sugars working group member; member of the Sugars  Fructans ERP; and member of the Codex Standard 234 AOAC Dairy  Methods ERP.  

YES – ADD TO THIS ERP

Salvatore Parisi 

Al Balqa Applied  University 

Research 

Academia 

Italy 

Statement of Expertise  I have taken part in the following activity   Standard Method Performance Requirements (SMPRs®) 2018.009: Lactose in Low‐Lactose or  Lactose‐Free Milk, Milk Products, and Products Containing Dairy Ingredients.  In addition, I am:   ‐ AOAC Methods in Codex STAN 234 Expert Review Panel (ERP) Member  ‐ AOAC SPSFAM Sugars Working Group member.  My researches concern low‐lactose products including cheeses, processed cheeses and dairy  products with vegetable fats.  Staff  Recommendation  YES – ADD TO THIS ERP  Has experience with analysis and chemistry of milk and milk products and  working group member for sugars.  Statement of Expertise  Chr. Hansen is one of the leading companies worldwide within the dairy ingredients sector. The  Department of Biochemical Analysis is providing analysis for Food and Enzyme R&D to develop  future products.  As the responsible scientist for the sugar analysis within Biochemical Analysis at  Chr. Hansen I am working on a daily basis with the analysis of lactose within all kind of different  matrices. Many of our laboratory activities are targeted the low‐lactose field and its challenges. My  expertise in this area covers a broad range of applied techniques, e.g. IC, enzyme‐based assays, LC‐ MS.  Staff  Recommendation  YES – ADD TO THIS ERP  Has experience with method development and analysis for dairy and sugar  analysis  Statement of Expertise  I have been involved in developing and upgrading nutrient analyses for 25+ years.  I served as chair  of the AOAC Sugars Working Group and on the Total Sugars, and Fructans ERPs.  My experience as a  Quality Manager for Yoplait included performing analyses for emergency issues and interpreting  data for proper application.  Staff  Has significant experience in method development and validation for dairy Angelika Semmler  Chr Hansen  Food  Production  Industry  Denmark  John Szpylka  Merieux NutriSciences CRO  Industry  USA 

Recommendation 

and sugar analysis.  Served as co‐chair for Sugars working group and is a  member of the Sugars Fructans ERP.   

YES – ADD TO THIS ERP 

Marina Torres 

LATU 

Research 

Academia 

Uruguay 

Statement of Expertise  I work developing and validating liquid chromatography sugar analysis (between others analytes) I  participating in different AOAC methods approval processes (ERPs member, working groups  member)  Staff  Recommendation  YES – ADD TO THIS ERP Has significant experience with method development and validation of  methods for infant formula and dairy and sugar analysis.  Served as a  working group member for the Sugars and SPIFAN FOS/GOS working  groups.  

Tomasz Tuzimski 

Medical University of  Lublin 

Research 

Academia 

Poland 

Statement of Expertise  I analyzed milk (cow's milk, soya's milk) and dairy products, and also human milk. I am currently  analyzing canned milk and human milk. I identify bisphenols.   Staff  Has significant experience with method development, milk analysis, and  pesticide analysis.   

Recommendation  POSSIBLE ADDITION

Martine van Gool 

FrieslandCampina 

Food  Production 

Industry 

Netherlands 

Statement of Expertise  Martine van Gool works as Analytical Researcher at FrieslandCampina, in the innovation centre in  Wageningen, The Netherlands. In this role she provides analytical expertise for R&D projects and to  support business decisions. She develops analytical methods for the analysis of (complex)  carbohydrates and proteins (including hydrolysates) that are present in diverse food matrices. Since  2015 she serves the AOAC‐SPIFAN nutrients ERP for GOS and FOS, the SPIFAN whey protein ERP and  since 2018 the SPSFAM sugar and fructan ERP.    Martine completed her PhD study in Food Chemistry (Wageningen University, The Netherlands) in  2012 and has experience with different analytical techniques such as UHPLC‐MS, MALDI‐TOF MS,  and various types of HPLC. 

Staff  Recommendation  YES – ADD TO THIS ERP

Has significant experience in method development and analysis of infant  formula and dairy products for sugars.  Served on the working group for  Sugars; member of SPIFAN Nutrient ERP for FOS/GOS; member of the  Sugar Fructans ERP.  

Sudhakar Yadlapalli 

First Source  Laboratory Solutions 

CRO 

Industry 

India 

Statement of Expertise  Established First Source laboratory Solutions LLP   (Analytical services) at Hyderabad, India. First  Source is one of the leading CRO in Hyderabad which was inspected by USFDA and also accredited by  NABL for ISO 17025 compliance. First Source support analytical testing requirements for major Food,  Agricultural and Pharmaceuticals industries in accordance to cGMP requirements.  I have an expertise in food safety and pharmaceutical analytical testing. Hands on experience on  high end mass spectrometers and   modern sample preparatory technology. Contributed in various  AOAC collaborative studies, expert review panels and working groups supported by AOAC

International.   Achieved AOAC Technical Excellence and Expert review panel award for the year 2016 and 2018  respectively. 

Staff  Recommendation  POSSIBLE ADDITION

Has experience in method validation and analytical testing methods for  infant formula and dairy products.  Serve as member of sugars working  group; Ethanol in Tea (Kombucha) ERP and working group.  

Jinchuan Yang 

Waters 

Instruments 

Industry 

USA 

Statement of Expertise  I have worked on the HPLC analysis method development on the carbohydrates and alcohols in the  past. Recently, I have been working on the mono‐ and disaccharides analysis for foods, and  published a number of application notes and posters.  

Staff  Recommendation  YES – ADD TO THIS ERP

Has significant expertise in method development and validation on  analysis for dairy products including carbohydrates analysis.  Served on the  sugars working group; SPIFAN Nutrients ERP, and Sugars Fructans ERP. 

All Applicant CVs (Next Page)

Curriculum Vitae (S. Austin)

Dr Sean Austin Sean Austin is a Research Scientist at Nestlé Research in Lausanne, Switzerland. His main responsibility is to develop and support analytical methods for the analysis of carbohydrate-based components in food and biological samples. In 16 years at Nestlé he has dealt with the analysis of mono-, oligo and polysaccharides, mostly using (U)HPLC, HPAEC-PAD, and LC-MS. Most recently his work has focused on the quantitative determination of oligosaccharides.

Education :

PhD - Structural characterisation of anti-nutritional polysaccharides in wheat

1992 - 1996

Nottingham University / Rowett Research Institute, UK

BSc (Hons) in Applied Chemistry (2:1) The Robert Gordon University, Aberdeen, UK

1987 - 1992

Employment:

Nestle Research Centre Vers-chez-les-Blanc, PO Box 44, CH-1000 Lausanne 26, Switzerland

Team Leader, AS Dept, “Carbohydrate Analytics” 01.02.2017 - Present Research Scientist, AS Pillar, “Proteins, Carbohydrates & Tracers” Group 01.04.2013 – 31.01.2017 Research Scientist, QS Dept, “Bioactives Analysis” Group 01.07.2012 – 31.03.2013 Research Scientist, QS Dept.,”Authenticity & Adulteration” Group 01.06.2010 – 30.06-2012 Research Scientist, QS Dept.,”Compound Identification” Group 01.04.2008 - 31.05.2010 Research Scientist, QS Dept., “Ingredients & Authenticity” Group 01.04.2003 - 31.03.2008

2000 - 2003

Bijvoet Centre for Biomolecular Characterisation

Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands . Post-Doc position working on the characterisation of glycoprotein glycans and bacterial capsular polysaccharides.

1997 - 2000

The National Institute For Biological Standards & Control

Blanche Lane, South Mimms, Potters Bar, Herts, UK. Post-Doc position working on a European collaborative project on the design and characterisation of glycoconjugate vaccines.

1996 - 1997

Rowett Research Institute

Greenburn Rd, Bucksburn, Aberdeen, UK. Temporary post working on the analysis of polysaccharides from differently processed grains.

1991

TR Oil Service

Kirkhill Industrial Estate, Dyce, Aberdeen, UK. Temporary post working in a lab to BS5750 standards checking the quality of incoming and outgoing chemicals.

1989 - 1990

Wiggins Teape Fine Papers Ltd

Stoneywood Mill, Bucksburn, Aberdeen, UK. Work experience placement from university for 1 year. Worked in the Technical Department on product development. Implemented statistical process control for some of the processes in the mill. Work was to BS5750 standards.

Analytical techniques :

Experienced in the use of many analytical techniques including: (U)HPLC-UV/FLD/RI/PAD, FPLC, (U)HPLC-ES-MS n , GC, GC-MS, MALDI-TOF-MS, NMR, 1D SDS-PAGE.

Organisations Member of the American Chemical Society Member of the Royal Society of Chemistry Member of AOAC International.

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Curriculum Vitae (S. Austin)

Publications :

Chesson, A. and Austin, S. (1998) Determining the nutritional characteristics of plant carbohydrates - the non-ruminant perspective. Proceedings of the 19th Western Nutrition Conference , University of Saskatehewan, Saskatoon, 205-214. Austin, S.C., Wiseman, J. and Chesson, A. (1999) Influence of non-starch polysaccharide structure on the metabolisable energy of UK wheat fed to poultry. Journal of Cereal Science . 29: 77-88. doi: 10.1006/jcrs.1998.0213 Lemercinier, X., Crane, D., Gee, C., Austin, S., Bolgiano, B., Jones, C. (1999) New methods for the characterisation of biopharmaceuticals: Conjugate Vaccines against Haemophilus influenzae type b. Developments in Biological Standardization. 101: 177-183.

Jones, C., Lemercinier, X., Crane, D.T., Gee, C.K., Austin, S. (2000) Spectroscopic studies of the structure and stability of glycoconjugate vaccines. Developments in Biologicals (Basel). 103: 121-136.

Austin, S., Koles, K., van Berkel, P., van Buuren, L., Kamerling, J. P. & Vliegenthart, J. F. G. (2001) Host- dependent glycosylation of a recombinant glycoprotein . Glycoconjugate Journal. 18: 123.

Bozzi, A., Perrin, C., Austin, S., Arce Vera, F. (2007) Quality and authenticity of commercial aloe vera gel powders. Food Chemistry. 103 : 22–30. doi:10.1016/j.foodchem.2006.05.061

Spichtig, V., Austin, S. (2008) Determination of the low molecular weight fraction of food-grade carrageenans. J. Chromatography B . 861 : 81-87. doi:10.1016/j.jchromb.2007.11.012

Cuany, D., Bénet, T., Austin, S. (2010) Development and single-laboratory validation of a method for the determination of total fructans in infant formula. J. AOAC Int. 93: 202-212

Spichtig, V., Michaud, J., Austin, S. (2010) Determination of sialic acids in milks and milk-based products. Anal. Biochem. 405 : 28-40. doi:10.1016/j.ab.2010.06.010

Cotting,C., Lardeau,A., Marmesat,S., Austin,S., Giuffrida,F. (2010) Quantification of glycolipids in cereal and dairy matrices. Lipid Technology 22 : 183-186. doi: 10.1002/lite.201000040 (Correction published Lipid Technology 22 : 222. doi: 10.1002/lite.201000062 ). Spichtig, V., Rohfritsch, P., Austin, S. (2011) Lactose does not interfere with the analysis of sialic acids as their 1,2-diamino-4,5-methylenedioxybenzene derivatives. Anal.Bioanal.Chem. 399 : 1917-1922. doi: 10.1007/s00216-010-4564-0.

Bénet, T., Austin, S. (2011) On-line cleanup for 2-aminobenzamide-labeled oligosaccharides. Anal.Biochem. 414 : 166-168. doi:10.1016/j.ab.2011.03.002

Austin, S., Spichtig, V. (2012) Response to Letter to the Editor regarding “Lactose does not interfere with the analysis of sialic acids as their 1,2-diamino-4,5-methylenedioxybenzene derivatives” . Anal.Bioanal.Chem. 404 : 921. doi: 10.1007/s00216-012-6154-9 Austin, S. , Benet,T., Michaud,J., Cuany,D., Rohfritsch, P., (2014) Determination of beta- galactooligosaccharides by Liquid Chromatography. Int.J.Anal.Chem. 2014 : Article ID 768406. doi:10.1155/2014/768406. Rostami,S.M., Benet,T., Spears,J., Reynolds,A., Satyaraj,E., Sprenger,N. (2014) Milk Oligosaccharides over Time of Lactation from Different Dog Breeds. PLoS ONE 9: e99824. doi:10.1371/journal.pone.0099824 Johansson,S., Diehl,B., Christakopoulos,P., Austin,S., Vafiadi,C. (2016) Oligosaccharide Synthesis in Fruit Juice Concentrates Using a Glucansucrase From Lactobacillus reuteri 180. Food and Bioproducts Processing 98 : 201-209. doi: 10.1016/j.fbp.2016.01.013 Austin,S.; De Castro,C.A.; Benet,T.; Hou,Y.; Sun,H.; Thakkar,S.K.; Vinyes-Pares,G.; Zhang,Y.; Wang,P. (2016) Temporal change of the content of 10 oligosaccharides in the milk of Chinese urban mothers. Nutrients , 8 : 349. doi: 10.3390/nu8060346

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Curriculum Vitae (S. Austin)

Brunt, K.; Sanders, P.; Spichtig, V.; Ernste-Nota, V.; Sawicka, P.; Iwanoff, K.; Van Soest, J.; Lin, P. K.; Austin, S. (2017) Dual-Laboratory Validation of a Method for the Determination of Fructans in Infant Formula and Adult Nutritionals: First Action 2016.14. J AOAC Int . 100 : 753-767. doi: 10.5740/jaoacint.17-0007 Srichuwong,S., Curti, D., Austin, S., King, R., Lamothe, L., Gloria-Hernandez, H. (2017) Physicochemical properties and starch digestibility of whole grain sorghums, millet, quinoa and amaranth flours, as affected by starch and non-starch constituents. Food Chem . 233 : 1-10. doi: 10.1016/j.foodchem.2017.04.019 Keller,S.R., Abonyi-Toth,Z., Sprenger, N., Austin, S.C., Wichert, B.A.C., Liesegang,A., Oei, C.H.Y., Balogh,O., Reichler,L.M. (2018) Effect of metoclopramide treatment of bitches during the first week of lactation on serum prolactin concentration, milk composition, and milk yield and on weight gain of their puppies. Am.J.Vet.Res. 79 : 233-241. doi: 10.2460/ajvr.79.2.233

Austin,S. Bénet,T.(2018) Quantitative determination of non-lactose milk oligosaccharides. Analytica Chmica Acta. 1010 : 86-96. doi: 10.1016/j.aca.2017.12.036

Austin,S., Cuany,D., Michaud,J., Diehl,B., Casado,B. (2018) Determination of 2’-fucosyllactose and lacto- N-neotetraose in infant formula. Molecules 23: 2650 doi: 10.3390/molecules23102650

Giuffrida,F., Austin,S., Cuany,D., Sanchez-Bridge,B., Longet,K., Bertschy,E., Sauser,J., Thakkar,S., Lee,L.Y., Affolter,M. (2018) Comparison of macronutrient content in human milk measured by mid-infrared human milk analyzer and reference methods. J. Perinatology . doi: 10.1038/s41372-018-0291-8 Sprenger,N., Binia,A., Austin,S. (2019) Human milk oligosaccharides: Factors affecting their composition and their physiological significance in Human Milk: Composition, Clinical Benefits and Future Opportunities. Nestlé Nutrition Institute Workshop Series. Vol 90 : 43-56. Donovan,S.M., German,J.B., Lönnerdal,B., Lucas,A. (eds), Karger/Nestlé Nutrition Institute, Basel, Switzerland. doi: 10.1159/000490292

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C U R R I C U L U M V I T A E

PERSONAL DETAILS Family Name:

DUCHATEAU

First Name:

Alexander, Lucia, Leonardus (nickname: Lucien)

Adres:

Smeetsstraat 83 3620 Lanaken (Belgium)

Date of Birth: Place of Birth Gender: Nationality:

2 March 1956

Maastricht (Netherlands)

Male

Dutch EDUCATION AND DEGREE Ph. D. in Chemistry (University Antwerp, Belgium, 1986) CAREER 1979-1980 :

researcher 'Interuniversitair Projekt voor Efficienter Kandidatuuronderwijs' (IPEK) Limburgs Universitair Centrum, Diepenbeek (Belgium) assistent-professor in Faculty Medicine Limburgs Universitair Centrum, Diepenbeek (Belgium)

1980-1985 :

1985-1988 :

scientist DSM Research, Geleen (Netherlands) group leader DSM Research, Geleen (Netherlands) senior scientist DSM Research, Geleen (Netherlands)

1988-1997 :

1997-2003:

2003-2011:

principal scientist chiral analysis DSM Innovative Synthesis B.V., Geleen (Netherlands)

2012-current:

principal scientist separations DSM Biotech Center, Delft (Netherlands)

ACTIVITIES/MEMBERSHIPS FOR SCIENTIFIC ORGANISATIONS/COMMITTEES/JOURNALS - Board member of the Section Analytical Chemistry of the Royal Flemish Chemical Society (KVCV) - Member of NEN committee of Dutch Normalisation Institute : 3702750013 “process contaminants” - Jury member of ‘COAST student Innovation Award’ (2016, 2017, 2018) -Referee of manuscripts for the following journals: Current Analytical Chemistry, Food Control, Food Chemistry - In 2011 and 2013, we participated with our in-house low lactose method in international proficiency testing of lactose free UHT milk organised by DRRR (Deutsches Referentzbüro für lebensmittel-Ringversuche und Referenzmateriallen) PUBLICATIONS IN PEER-REVIEWED JOURNALS Total number: 53 Topics covered in publications: liquid-and gas chromatographic separation of metabolites (e.g. amino acids, neurotransmittors, sugars). See below recent publication on lactose: Wibo B. van Scheppingen, Piet H. van Hilten, Marieke P. Vijverberg, Alexander L.L. Duchateau Selective and sensitive determination of lactose in low-lactose dairy products with HPAEC-PAD, J. Chromatogr. B, 1060, 395-399 (2017)

RM4.5022 Version: 1 Effective Date: 06.12.2018 Approved By: CZ4T, J9C2, 22.10.2018

CURRICULUM VITAE: DAVID J. ELLINGSON

CURRENT POSITION

Title:

Manager, Global Laboratory Performance Leader - Vitamins

Department:

Chemistry Vitamins Eurofins Food Integrity & Innovation Madison, WI

EDUCATION

MCPM Master’s Certificate in Project Management, University of Wisconsin - Madison School of Business, Madison, WI 2017 MS Cellular and Molecular Biology, University of Wisconsin - La Crosse, La Crosse, WI 2007 BS Biology and Zoology, University of Wisconsin - Madison, Madison, WI 2004

Certifications

Six Sigma Yellow Belt Certification, University of Wisconsin - Madison School of Business 2016 Ion Chromatography Method Development, Bannockburn, IL, 2011 Dionex ICS3000: Operation, Maintenance, and Troubleshooting, Bannockburn, IL, 2008

PROFESSIONAL EXPERIENCE

12/2018 – Present: Manager, Global Laboratory Performance Leader – Vitamins Mr. Ellingson is currently responsible for the performance of the Vitamins operational groups in Madison, WI, Battle Creek, MI, Harrogate, UK, and Singapore Laboratories. These responsibilities include oversight of the data, science and methodology, instrumentation, personnel and resources needed to meet EFII performance requirements and any client needs. 06/2017 – 12/2018: Manager, Laboratory Performance Leader – Specialty Testing Mr. Ellingson is currently responsible for the performance of the Special Analysis and Monograph operational groups in Madison, WI and the Brea, CA Laboratory. These responsibilities include oversight of the data, science and methodology, instrumentation, personnel and resources needed to meet CFS performance requirements and any client needs. 08/2015 – 06/2017: Senior Research Chemist, Method Development Mr. Ellingson served as the Covance Food Solutions (CFS) project manager for implementation of the Chromeleon Chromatography Data System (CDS). He was responsible for organization, activities, and successful completion of this implementation. In addition, he still maintained responsibilities below. 07/2013 – 08/2015: Research Chemist II, Technical Development In addition to Research Associate I responsibilities, Mr. Ellingson was responsible for development of new methods for NCFS that were novel or that replaced existing methods. He independently served as lead scientist for multiple development projects and validations. He oversaw technical staff involved to design and/or guide experiments, and ensured technical soundness with completion in a timely manner. Mr. Ellingson also trained technical staff on use of instrumentation and chemistry involved in a variety of analytical techniques and disciplines.

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RM4.5022 Version: 1 Effective Date: 06.12.2018 Approved By: CZ4T, J9C2, 22.10.2018

09/2011 – 07/2013: Research Associate I, Technical Development Mr. Ellingson was responsible for development of new methods, improving current methods, and development and transfer of client methods to their respective groups within NCFS. He served as a resource for scientific background in a variety of different analytical techniques and disciplines, and also served as a resource to identify and resolve issues that arose within those areas. He served as the main resource for all Ion Chromatography (IC) methods and development. He continued to be involved in scientific associations, and presented and published new analytical advancements he developed. 04/2009 – 08/2011: Research Assistant III, Nutritional Chemistry and Food Safety In addition to Research Assistant II responsibilities below, Mr. Ellingson continued responsibilities as IC leader for the department. He also took on responsibility as a technical resource for client inquiries regarding analysis of functional fibers and capabilities for future work on the IC systems. He developed IC methods that eliminated the need for older assays run by Gas Chromatography (GC) and Liquid Chromatography (LC) equipment, and was a technical resource for others in the lab. 07/2008 – 03/2009: Research Assistant II, Food and Drug Analysis Mr. Ellingson was responsible for all IC assays, systems, and maintenance. He was responsible for multiple Good Laboratory Practice (GLP) studies run by IC. He was also involved in assay development for both High Performance Liquid Chromatography (HPLC) and IC assays. He was highly skilled in the use of all standard laboratory equipment, HPLC/IC systems, and GLP documentation, and was proficient in the LabWare Information Management System (NIMS) software. 04/2007 – 06/2008: Research Assistant I, Food and Drug Analysis Mr. Ellingson was responsible for multiple assays in compliance with Standard Operating Procedures (SOPs), applicable methods, and regulatory agency guidelines. Mr. Ellingson used the Laboratory Information Management System (LIMS), the LIMS Access Tool (LAT), and Empower applications. He was proficient in multiple HPLC systems and helped troubleshoot issues and implement assays. He was highly skilled in the use of all standard laboratory equipment, and maintained well-documented laboratory records. 09/2004 – 12/2007: Master’s Thesis, University of Wisconsin – La Crosse, La Crosse, WI Mr. Ellingson completed his Master Thesis, “Meiotic Protein Expression and Interactions of Yeast Cell Cycle Gene CDC7 ”.

09/2005 – 12/2006: Teaching Assistant, University of Wisconsin – La Crosse, La Crosse, WI Mr. Ellingson was an introductory Biology lab teacher.

05/2004 – 08/2004: 3M Engineering Assistant, Menomonie, WI Mr. Ellingson created new standard operating procedures manual for a new product line, and utilized statistics to troubleshoot issues on existing product lines.

PUBLICATIONS AND PRESENTATIONS

Ellingson, D., Galbraith, A. “Meiotic Protein Expression and Interactions of Yeast Cell Cycle Gene CDC7 ,” Yeast Genetics and Molecular Biology Conference, Princeton University, Princeton, NJ, July 2006.

Ellingson, D. “An Overview of Dietary Fiber Analysis with a Concentration on Functional Fiber,” oral presentation, Madison, WI, March 2009.

Ellingson, D., Burkhardt, G., Ross, L., Winters, D., Sullivan, D. “Quantitative Analysis of Sugar Alcohols by HPAEC-PAD,” AOAC International Annual Meeting, Philadelphia, PA, September 2009.

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RM4.5022 Version: 1 Effective Date: 06.12.2018 Approved By: CZ4T, J9C2, 22.10.2018

Rozema, B., Ellingson, D., Zielinski, G., Fladhammer, J. “Dietary Fiber Applications Across a Variety of Matrices ,” video presentation, Madison, WI, June 2010.

Ellingson, D., Potts, B., Anderson, P., Burkhardt, G., Ellefson, W., Sullivan, D., Jacobs, W., Ragan, R. “Improved Method for the Direct Determination of Available Carbohydrates in Low Carbohydrate Products Using High-Performance Anion Exchange Chromatography,” AOAC International Annual Meeting, Orlando, FL, September 2010. Ellingson, D., Potts, B., Anderson, P., Burkhardt, G., Ellefson, W., Sullivan, D., Jacobs, W., Ragan, R. “Method for the Direct Determination of Available Carbohydrates in Low Carbohydrate Products Using High-Performance Anion Exchange Chromatography,” Journal of AOAC International Vol. 93, No. 6, 2010. Ellingson, D., Pritchard, T., Foy, P., King, K., Mitchell, B., Winters, D., Sullivan, D. “Analysis of Free and Total myo-Inositol in Foods, Feeds, and Infant Formulas by Microwave Digestion Followed by Enzymatic Treatment and Quantitation by HPAEC-PAD,” AOAC International Annual Meeting, New Orleans, LA, September 2011. Ellingson, D., Pritchard, T., Foy, P., King, K., Mitchell, B., Winters, D., Sullivan, D. “Analysis of Free and Total myo-Inositol in Foods, Feeds, and Infant Formulas by Microwave Digestion Followed by Enzymatic Treatment and Quantitation by HPAEC-PAD,” Journal of AOAC International Vol. 95, No. 5, 2012. Ellingson, D., Pritchard, T., Foy, P., King, K., Mitchell, B., Winters, D., Sullivan, D. "Analysis of Free and Total Myo-Inositol in Infant Formula and Adult/Pediatric Nutritional Formula by HPAEC-PAD, Including a Novel Total Extraction Using Microwave-Assisted Acid Hydrolysis and Enzymatic Treatment: First Action 2012.12", Journal of AOAC International Vol. 96, No.5, 2013. Ellingson, D., Zywicki, R., Sullivan, D. “Analytical Method for the Determination of Various Arsenic Species in Rice, Rice Food Products, Apple Juice, and Other Juices by Ion Chromatography-Inductively Coupled Plasma/Mass Spectrometry”, Journal of AOAC International Vol. 97, No. 6, 2014.

Ellingson, D. “The Development of a Method for Arsenic Speciation and the Use of Ion Chromatography as a Solution for Separation”, LC/GC webinar sponsored by Thermo Scientific, October 2014.

Ellingson, D., Shippar, J., Gilmore, J. “Determination of Free and Total Choline and Carnitine in Infant Formula and Adult/Pediatric Nutritional Formula by Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS): First Action 2015.10”, Journal of AOAC International Vol. 98, No. 7, 2015. Ellingson, D., Anderson, P., Berg, D. “Analytical Method for Sugar Profile in Pet Food and Animal Feeds by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection”, Journal of AOAC International Vol. 99, No. 2, 2016.

Ellingson, D. “Insights into Challenges of Labeling Added Sugar”, Global Food Forums 2016 Sweetener Systems Trends and Technologies Conference, Podium Presentation, Chicago, IL, November 2016.

Ellingson, D. “A Method for Food, Supplements and Animal Feeds using High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection”, Separation Science webinar sponsored by Thermo Scientific, June 2017. Vennard, T., Ruosch, A., Ellingson, D., Berg, D, Mastovska, K. “A Multi-Laboratory Validation of an Optimized Sugar Profile Method in a Variety of Matrices Using High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD),” AOAC International Annual Meeting, Atlanta, GA, September 2017.

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RM4.5022 Version: 1 Effective Date: 06.12.2018 Approved By: CZ4T, J9C2, 22.10.2018

Ellingson, D., Shippar, J., Vennard, T., Moloney, C., O’Connor, D., O’Regan, J., McMahon, A., Affolter, M., “Analytical Method for Lactoferrin in Milk-Based Infant Formulas by Signature Peptide Quantification with Ultra-High Performance LC-Tandem Mass Spectrometry,” Journal of AOAC International, “In Press”.

PROFESSIONAL MEMBERSHIPS AND AFFILIATIONS

AOAC member AOAC SPIFAN and SPSFAM working group member (multiple working groups)

Signed by the employee (to confirm accuracy of this record): ________________________________________

Document formally issued: ____________________ Date superseded: _________________________

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Curriculum Vitae

Nour-Eddine ES-SAFI Date of birth: 04/20/1962 Place of birth: Morocco. ADDRESS

Team of Organic Chemistry and Physical and Chemical Studies. Mohammed V- University in Rabat EcoleNormaleSupérieure B.P 5118 Takaddoum, Rabat, Morocco. Ph. : +212 5 37 75 80 96. Mobile: +212 6 72 64 30 86. Fax.: +212 5 37 75 90 63. E-mail: nouressafi@yahoo.fr nour-eddine.es-safi@um5.ac.ma Web: https://www.researchgate.net/profile/Nour_Eddine_Es-Safi https://scholar.google.com/citations?user=Bpit1e4AAAAJ&hl=en https://www.linkedin.com/in/nour-eddine-es-safi-aa3b6a14/ ORCID ID: 0000-0001-9903-8129 Researcher ID: A-3541-2008 Scopus Author ID: 6701924280

ACADEMIC EDUCATION 1997 : Ph.D in Organic Chemistry. Mohammed V University inRabat, Morocco. Thesis entitled “ Phenolic compounds of red vine ( Vitis vinifera L.) cultivated in Morocco: Structure, reactivity and pharmacological properties ”. 1990 : Master of Science in Organic Chemistry. Ecole Normale Supérieure, Rabat, Morocco. Thesis entitled “ Contribution to the study of essential oils and tanins of Eucalyptus trees cultivated in Morocco ” 1986 : Certificate for teaching chemistry in training centers frames. Ecole Normale Supérieure Rabat, Morocco. 1984 : Graduate of the Ecole Normale Supérieure, Option Physique – Chimie, Rabat, Morocco.

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EMPLOYMENT July 2014-Present: Deputy Director of the Ecole Normale Supérieure. Mohammed V University in Rabat – Morocco. Jan 2001-Present: Professor at Mohammed V University in Rabat. Ecole Normale Supérieure, Rabat – Morocco (Chemisty department). Jan 1997- 2001: Associate Professor at the same department. 1986-1997 : Assistant Professor at the same department. 1984-1986 : Assistant at the same department. PROFESSIONAL EXPERIENCE Research Competence:  Expertise in synthesis of natural bioactive molecules.  Dexterous in isolation and characterization of natural bioactive molecules.  Endured in minor natural products purification.  Familiar with HPLC and related chromatographic techniques.  Adept in analyzing spectral data like 1 H, 13 C, 2D NMR (COSY, NOESY, HMBC and HMQC), IR and MS (ESI-MS, Tandem MS-MS, Maldi-Tof MS).  Design and synthesis of biologically active compounds.  Development of synthetic methodologies for natural/modified compounds synthesis.  Isolation and characterization of naturally occurring bio-active compounds, hemisynthesis of isolated natural products for the confirmation of their chemical structures, transformations of natural products into their novel derivatives.  Isolation of novel compounds (polyphenols, iridoids, phenylethanoids) from Moroccan plants.  Development of regio- and stereoselective methodologies for modified polyphenols synthesis.  Development of thioacidolysis method for structural elucidation of bridged flavanols.  Capable of both collaborative and independent research.  Possession of good communication and management skills.

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Research Conducted or Supervised  Natural Products: 

Essential oils, polyphenols, iridoids  Eucalyptus trees: Extraction, quantitative and qualitative analysis of essential oils (leaves) and condensed tannins (bark).  Vine leaves polyphenols (proanthocyanidins, flavonols, anthocyanidins): Extraction, quantitative analysis, isolation and identification by spectroscopic tools (mass and 1D and 2D NMR spectroscopy).  Phytochemical investigations of various Moroccan medicinal plants used in traditional pharmacopeae.  Analytical methods:  Chromatography: HPLC/DAD, HPLC/ESI-MS. Application to the study of the condensation of flavanols and anthocyanins in model solution systems.  Spectroscopy: ESI/MS, MS/MS, ICD MS, 1D ( 1 H, 13 C) and 2D (COSY, HSQC, HMBC, NOESY, ROESY) NMR techniques. Application to the structural elucidation of natural and synthesized compounds.  Composition of foods:  Grape proanthocyanidins: Extraction from different parts (seeds, stems and skins), quantitative and qualitative analysis by HPLC and cleavage methods (thiolacidoysis).  Grapeanthocyanins: Extraction, quantitative and qualitative analysis.  Food polyphenols and their role in human health  Polyphenols and their role in food technology  Flavor and aroma:  Essential oils: extraction, analysis by GC/MS techniques, antibacterial and antifungal properties.

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 Chemical changes during processing:  Chemical transformations which contribute to the organoleptic properties alterations (de-astringency, browning, darkening, etc …) of foods during processing, maturation, storage and aging.  Evolution of phenolic compounds during processing, maturation, storage and aging of foods in model solution systems.  Evolution of phenolic compounds in model solution systems. Detection, isolation and identification of the new formed compounds and confirmation of their presence in in foods.  Role of the new formed compounds in food organoleptic properties and huaman health.  Pharmacological activity:  Antibacterial and antifungal activity of natural and synthesized compounds.  Mollusciscidal activity of proanthocyanidols and flavonols isolated from grapes and vine leaves.  Antioxydant and free radical scavenging properties of natural and synthesized compounds.  Strucure-activity relationships. Teaching Experience  Organic Chemistry: Stereochemistry, reactions, mechanisms and structures.  Natural Products.  Spectral Analysis: IR, MS, NMR.  Chromatography: PC, GPC, HPLC.  Polyphenols chemistry  Scientific communication  Valorization of plants in the cosmetic sector  Valorization of plants in the agro-food sector  Valorization of plants in the pharmaceutic sector

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Scientific stays in foreign laboratories:  Unité de Phytopharmacie et Médiateurs Chimiques. Team: Chemistry of Natural Products. National Institute for Agricultural Research, Versailles, France.  UMR Sciences for Enology. Polyphenols team. National Institute for Agricultural Research, Montpellier, France.  Laboratory of Polyphenols. National Institute for Agricultural Research, Narbonne, France.  MateriaMedica and Pharmacognosy Laboratory, School of Pharmacy, Montpellier, France.. LANGUAGES  English, French and Arabic (Native Language): Writing, Speaking and Reading. MICROCOMPUTER USE Deputy Director of Ecole Normale Supérieure, Rabat, Morocco. Since July 2014.  Head of the Chemistry department at EcoleNormale Supérieure, Rabat, Morocco. 2011-2014.  Professor at EcoleNormale Supérieure,Mohammed V University of Rabat. Since 2001.  Responsibleof the research team "Organic Chemistry and Physical and Chemical Studies".  Responsible of the Research Master "Valorization of the natural resources plant".  Responsible of the professional bachelor's degree "university course teaching in physics and chemistry".  Project Expert Reviewer of the Chilean National Science and Technology Commission (CONICYT-Chile)  Expert Review Panel for the AOAC Stakeholder Panel on Strategic Food Analytical Methods (Flavanols, St. John's Wort, Chondroitin, Kratom, Ashwagandha, Folin C, …) OTHERS 

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Curriculum Vitae - N. ES-SAFI - March 10, 2019. ) Phenolic Composition, Antioxidant, Cytotoxicity and Anti-Inflammatory Activities of Extracts Obtained From Germinated and Crude Fenugreek ( Trigonella foenum-graecum ) Seeds. In:Govil, J.N. and Singh, S. (eds) Recent Progress in Medicinal Plants. Vol. 46 Metabolic Disorder Diabetes, Part II. Studium Press, LLC, USA, pp. 401-422. 6. BEN AJMIA, W.; MAKNI, M.; AMMAR, S.; KHANNOUS, L.; BEN HASSANA, A.; BOUAZIZ, M.; E 10.1080/10496475.2018.1556372 S-SAFI, N .; GDOURA, R. ( 2017 ).  Expert Review Panel for the AOAC Stakeholder Panel on Dietary Supplements  Member of various scientific associations.  Post-doc at the French National Agricultural Research Institute. Montpellier & Versailles INRA centers.  Supervisor of PhD students.  Editor in Chief of Green and Sustainable Chemistry journal  Regional Editor of the American Journal of Food Technology.  Guess Editor of Current Bioactive Compounds journal special issue on "Current Hyphenated Methods in Polyphenol Analysis".  Editorial Board Member of various scientific journals  Reviewer for many scientific journals  Author of many publications in various scientific journals Publications (since 2000) 1. GUAOUGUAOU, F.; TAGHZOUTI, K.; OUKABLI, M.; MASRAR, A.; CHABRAOUI, L.; BOUABDELLAH, M.; ES-SAFI N. (2018) Acute and Subchronic Oral and Dermal Toxicological Studies of Salvia Verbenaca Extracts in Mice and Rats . Journal of Herbs, Spices & Medicinal Plants, In Press (DOI: 10.1080/10496475.2018.1556372 ) 2. GUAOUGUAOU, F.; AHL BEBAHA, M. A.; TAGHZOUTI, K.; ES-SAFI, N. ( 2018 ) Phytochemical Investigation, Acute Toxicity, Central Analgesic and Antioxidant Activities of Extracts and Essential Oil of Cotula cinerea Del (Asteraceae) . Current Bioactive Compounds , In Press (DOI : 10.2174/1573407214666180821115826) 3. GUAOUGUAOU, F.; AHL BEBAHA, M. A.; TAGHZOUTI, K.; BOUYAHYA, A.; BAKRI, Y.; DAKKA, N.; ES-SAFI, N. ( 2018 ) Cytotoxicological Investigation of the Essential Oil and the Extracts of Cotula cinerea and Salvia verbenaca from Morocco. BioMed Research International , 2018, Article ID 7163961, 5 pages https://doi.org/10.1155/2018/7163961. 4. GUAOUGUAOU, F.; TAGHZOUTI, K.; OUKABLI, M.; ES-SAFI, N. ( 2018 ) The effect of Salvia verbenaca extracts for healing of second-degree burn wounds in rats . Current Bioactive Compounds , 14, 419-427. 5. BENAYAD, Z.; GOMEZ-CORDOVES, C.; ES-SAFI, N. ( 2018

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